parzival · 2021-06-15T13:38:35Z Video: Roger Waters Tells “Little Prick” Zuckerberg To “F*** Off” Following Request To Use Iconic Pink Floyd Song For Ad [ Link ]
parzival · 2021-06-15T13:38:35Z
Video: Roger Waters Tells “Little Prick” Zuckerberg To “F*** Off” Following Request To Use Iconic Pink Floyd Song For Ad [ Link ]
Joyly · 2021-06-16T21:31:48Z parzival It is great to see people like Roger Waters who are not for sale - at any price - putting ethics before profits. Fauci will likely spend a thousand years in hell before he sees that lesson.
parzival It is great to see people like Roger Waters who are not for sale - at any price - putting ethics before profits. Fauci will likely spend a thousand years in hell before he sees that lesson.
LindaMay · 2021-06-15T14:41:43Z Check his chart out. [ Link ] [ Link ] [ Link ]
LindaMay · 2021-06-15T14:41:43Z
Check his chart out. [ Link ] [ Link ] [ Link ]
Baybars · 2021-06-16T19:27:02Z LindaMay Good links. I enjoyed reading them. Thank you
Baybars · 2021-06-16T19:27:02Z
LindaMay Good links. I enjoyed reading them. Thank you
LindaMay · 2021-06-16T19:45:00Z Baybars 🙇♀️ So good to see you B
LindaMay · 2021-06-16T19:45:00Z
Baybars 🙇♀️ So good to see you B
parzival · 2021-06-15T18:19:44Z 'BiG DXXCHe IS WaTCHiNG YoU' [ Link ]
parzival · 2021-06-15T18:19:44Z
'BiG DXXCHe IS WaTCHiNG YoU' [ Link ]
ReRan · 2021-06-15T23:06:11Z This is a long show, but well worth it. [ Link ] I'm not good at links, hope it works. The Highwire, episode 219.
This is a long show, but well worth it. [ Link ] I'm not good at links, hope it works. The Highwire, episode 219.
parzival · 2021-06-16T02:16:48Z ReRan Greatt Link to a Great Show! Thanks !!
parzival · 2021-06-16T02:16:48Z
ReRan Greatt Link to a Great Show! Thanks !!
Tsidkenu · 2021-06-16T15:03:05Z ReRan Dang, Yeadon does well (would be nice if he had a chat to Stefan Lanka though!) Summarising one of the more important parts of Yeadon's discussion, there are three fundamental questions explaining why reactions to the shank are so varied: 1. Where does the shank end up in the subject's body? A person whose shank remains mostly in the muscle cells of their arm will be less affected than one whose shank travels the circulatory system to the brain or heart (or elsewhere). 2. How much of the shank is taken up by the cells in the subject's body? A person whose shank is widely taken up by their cells, for whatever reason, will have radically different outcome to someone whose shank its not widely taken up by the cells. 3. What amount of 'spike protein' is consequently manufactured by the cells? Even after taking up the production code (mRNA or adenovirus vector), a cell might produce a little or a lot of 'spike protein'. When combined with location, this can have mild to fatal results. Yeadon notes that all three points are entirely absent from any and all safety discussions of the shanks, irregardless of manufacturer.
Tsidkenu · 2021-06-16T15:03:05Z
ReRan Dang, Yeadon does well (would be nice if he had a chat to Stefan Lanka though!) Summarising one of the more important parts of Yeadon's discussion, there are three fundamental questions explaining why reactions to the shank are so varied: 1. Where does the shank end up in the subject's body? A person whose shank remains mostly in the muscle cells of their arm will be less affected than one whose shank travels the circulatory system to the brain or heart (or elsewhere). 2. How much of the shank is taken up by the cells in the subject's body? A person whose shank is widely taken up by their cells, for whatever reason, will have radically different outcome to someone whose shank its not widely taken up by the cells. 3. What amount of 'spike protein' is consequently manufactured by the cells? Even after taking up the production code (mRNA or adenovirus vector), a cell might produce a little or a lot of 'spike protein'. When combined with location, this can have mild to fatal results. Yeadon notes that all three points are entirely absent from any and all safety discussions of the shanks, irregardless of manufacturer.
DeliciousTears · 2021-06-16T17:57:10Z Tsidkenu From Foia type documents of studies run by Big Harma (Pfizer I think), there were detectable levels of spike protein in the blood of shanking victims anywhere from 2 to 29 days in a sample of 20k. So it varies widely and that's the fox reporting on the henhouse.... Soak human ovaries in shank spikes for 20+ days and what happens? Probably nothing good .
DeliciousTears · 2021-06-16T17:57:10Z
Tsidkenu From Foia type documents of studies run by Big Harma (Pfizer I think), there were detectable levels of spike protein in the blood of shanking victims anywhere from 2 to 29 days in a sample of 20k. So it varies widely and that's the fox reporting on the henhouse.... Soak human ovaries in shank spikes for 20+ days and what happens? Probably nothing good .
Rowan Cocoan · 2021-06-17T04:27:29Z ReRan In my experience, the way to make links work here is just before you paste it, hit the space bar, and after you paste it, hit the space bar. RC
Rowan Cocoan · 2021-06-17T04:27:29Z
ReRan In my experience, the way to make links work here is just before you paste it, hit the space bar, and after you paste it, hit the space bar. RC
parzival · 2021-06-17T07:31:08Z You Know It’s Bad When a NORTH KOREAN DEFECTOR Says That US Similarities to NK Are “Insane” [ Link ]
parzival · 2021-06-17T07:31:08Z
You Know It’s Bad When a NORTH KOREAN DEFECTOR Says That US Similarities to NK Are “Insane” [ Link ]
ПКостов · 2021-06-17T16:14:22Z WE ARE AT WAR - DR. TENPENNY, DR. PALEVSKI, DR. MADEJ (JUNE 10, 2021) [ Link ]
ПКостов · 2021-06-17T16:14:22Z
WE ARE AT WAR - DR. TENPENNY, DR. PALEVSKI, DR. MADEJ (JUNE 10, 2021) [ Link ]
Nada · 2021-06-17T17:17:55Z For anyone interested, Richard Grove & Co. of “Grand Theft World” engage in comprehensive analysis when it comes to understanding the truth of the realities we experience. [ Link ] Here’s Episode 30 titled “Fauci Caught Wuhanded”
For anyone interested, Richard Grove & Co. of “Grand Theft World” engage in comprehensive analysis when it comes to understanding the truth of the realities we experience. [ Link ] Here’s Episode 30 titled “Fauci Caught Wuhanded”
Rowan Cocoan · 2021-06-17T17:54:42Z Nada Fauci Caught Wuhanded” RC
Rowan Cocoan · 2021-06-17T17:54:42Z
Nada Fauci Caught Wuhanded” RC
Tsidkenu · 2021-06-17T22:51:49Z Vax nanotech gathers in body organs according to mRNA vax inventor Robert Malone: [ Link ]
Tsidkenu · 2021-06-17T22:51:49Z
Vax nanotech gathers in body organs according to mRNA vax inventor Robert Malone: [ Link ]
Nada · 2021-06-20T03:11:41Z Tsidkenu I would love to ask this guy one question. “Why The fuck did you invent this evil tech in the first place?”
Tsidkenu I would love to ask this guy one question. “Why The fuck did you invent this evil tech in the first place?”
DeliciousTears · 2021-06-20T15:52:23Z Nada MRNA tech has been around for a long while now. It's primary usage with some great success has been for treatment of very serious/fatal cancers. I have a friend who found out she had 4th stage melanoma (at 47 years old). Essentially she was dead. They used some super-experimental mrna vehicle to deliver the treatment and she went into complete remission. She was 100% fine for over a year until the auto-immune stuff kicked in. Long story short... she's now has had her colon removed, can't eat foods, and exists entirely on TPN... IV dripped nutrients... So the tech does have promise, but for serious/fatal diseases on a case by case evaluation. Obviously not something for a survival rate over 99% and into millions of people in an unregulated and illegal phase 3 trial.
DeliciousTears · 2021-06-20T15:52:23Z
Nada MRNA tech has been around for a long while now. It's primary usage with some great success has been for treatment of very serious/fatal cancers. I have a friend who found out she had 4th stage melanoma (at 47 years old). Essentially she was dead. They used some super-experimental mrna vehicle to deliver the treatment and she went into complete remission. She was 100% fine for over a year until the auto-immune stuff kicked in. Long story short... she's now has had her colon removed, can't eat foods, and exists entirely on TPN... IV dripped nutrients... So the tech does have promise, but for serious/fatal diseases on a case by case evaluation. Obviously not something for a survival rate over 99% and into millions of people in an unregulated and illegal phase 3 trial.
Rowan Cocoan · 2021-06-20T17:29:38Z DeliciousTears Wow. Initially, I was stoked to hear how it went, and sorry to hear the later stuff. Thank you! RC Nada, infra: The theme is seems to be prevalent. Things that can help society and humanity as a whole get corrupted, perverted, and inverted for the sake of power and control. You add a holistic view. Excellent! RC
Rowan Cocoan · 2021-06-20T17:29:38Z
DeliciousTears Wow. Initially, I was stoked to hear how it went, and sorry to hear the later stuff. Thank you! RC Nada, infra: The theme is seems to be prevalent. Things that can help society and humanity as a whole get corrupted, perverted, and inverted for the sake of power and control. You add a holistic view. Excellent! RC
The theme is seems to be prevalent. Things that can help society and humanity as a whole get corrupted, perverted, and inverted for the sake of power and control.
Nada · 2021-06-20T17:30:37Z DeliciousTears The theme is seems to be prevalent. Things that can help society and humanity as a whole get corrupted, perverted, and inverted for the sake of power and control.
DeliciousTears The theme is seems to be prevalent. Things that can help society and humanity as a whole get corrupted, perverted, and inverted for the sake of power and control.
LindaMay · 2021-06-20T18:32:55Z DeliciousTears having an astrologist look at her natal chart would definately bring more insight to ponder.
LindaMay · 2021-06-20T18:32:55Z
DeliciousTears having an astrologist look at her natal chart would definately bring more insight to ponder.
Nada · 2021-06-21T00:45:16Z LindaMay Sounds interesting. Where can I deep dive into this sort of info? Any charts on the powers that wish they were and wether or not humanity will fall for the rat trap they’ve created?
LindaMay Sounds interesting. Where can I deep dive into this sort of info? Any charts on the powers that wish they were and wether or not humanity will fall for the rat trap they’ve created?
LindaMay · 2021-06-21T01:28:25Z Nada we're falling lolll But some have crocheted a set of wings. What exactly interests you Nada, daily or weekly insights. Or both?
LindaMay · 2021-06-21T01:28:25Z
Nada we're falling lolll But some have crocheted a set of wings. What exactly interests you Nada, daily or weekly insights. Or both?
Nada · LindaMay Both. · 2021-06-21T21:41:07Z
LindaMay · 2021-06-21T22:00:35Z Nada okkk These are greatly on the ball!! Daily [ Link ] Weekly [ Link ] [ Link ] Stormie has a year-full of Eat n Greets, amazing videos. She also initiated a free year-long bi-weekly Astrology Youtube University, do at your pace courses. EXTRA GREAT. Called KICKSTARTERS FullMoon/NewMoon Astrology [ Link ] Brilliant Divination [ Link ] [ Link ]
LindaMay · 2021-06-21T22:00:35Z
Nada okkk These are greatly on the ball!! Daily [ Link ] Weekly [ Link ] [ Link ] Stormie has a year-full of Eat n Greets, amazing videos. She also initiated a free year-long bi-weekly Astrology Youtube University, do at your pace courses. EXTRA GREAT. Called KICKSTARTERS FullMoon/NewMoon Astrology [ Link ] Brilliant Divination [ Link ] [ Link ]
parzival · 2021-06-21T01:47:36Z The amplification of STUPID : 'A man who boosted his Pfizer COVID-19 vaccine with a shot from Moderna said his side effects were a 'little more severe' the third time round' [ Link ]
parzival · 2021-06-21T01:47:36Z
The amplification of STUPID : 'A man who boosted his Pfizer COVID-19 vaccine with a shot from Moderna said his side effects were a 'little more severe' the third time round' [ Link ]
Rowan Cocoan · 2021-06-21T02:21:06Z parzival A year ago, I would have said, OMG! Now I just shrug. RC
Rowan Cocoan · 2021-06-21T02:21:06Z
parzival A year ago, I would have said, OMG! Now I just shrug. RC
parzival · 2021-06-21T02:27:30Z Rowan Cocoan It's so ridiculous that this has become normalised...
parzival · 2021-06-21T02:27:30Z
Rowan Cocoan It's so ridiculous that this has become normalised...
Rowan Cocoan · 2021-06-21T02:29:36Z parzival There's a better way to say this (which is related to how reality has overrun satire) but it's like reality is multiplied by -1? -1/2? That it (satire) has effectively been destroyed or at least neutered. RC
Rowan Cocoan · 2021-06-21T02:29:36Z
parzival There's a better way to say this (which is related to how reality has overrun satire) but it's like reality is multiplied by -1? -1/2? That it (satire) has effectively been destroyed or at least neutered. RC
parzival · 2021-06-21T02:36:16Z Rowan Cocoan COVID Clown World...
parzival · 2021-06-21T02:36:16Z
Rowan Cocoan COVID Clown World...
smertzasmert · 2021-06-21T03:11:56Z the concept of "objective health" is ludicrous many including Foucault demonstrated that concepts of illness, sexualtiy, power are culturally determined
smertzasmert · 2021-06-21T03:11:56Z
the concept of "objective health" is ludicrous many including Foucault demonstrated that concepts of illness, sexualtiy, power are culturally determined
Rowan Cocoan · 2021-06-21T04:46:13Z smertzasmert Now you're merely dealing with semantics. RC
Rowan Cocoan · 2021-06-21T04:46:13Z
smertzasmert Now you're merely dealing with semantics. RC
smertzasmert · 2021-06-21T08:05:18Z Rowan Cocoan now you are insane...why are north amerikan definitions of diseases not employed in Europe? why has the DSM been changed 8 times and is employed in civilized nations? why do amerikans complain about pain more than any people? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? why do only north amerikans have this as Geoffrey Gorer wrote "a patterned courtship ritual described as dating where they are required to meet in public spaces several occasions before they copulate"?
smertzasmert · 2021-06-21T08:05:18Z
Rowan Cocoan now you are insane...why are north amerikan definitions of diseases not employed in Europe? why has the DSM been changed 8 times and is employed in civilized nations? why do amerikans complain about pain more than any people? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? why do only north amerikans have this as Geoffrey Gorer wrote "a patterned courtship ritual described as dating where they are required to meet in public spaces several occasions before they copulate"?
Rowan Cocoan · 2021-06-21T08:28:44Z smertzasmert I said, 'Now you're merely dealing with semantics . and you reply with now you are insane... Well, from around here, we'd say something like: You need to work on your art of debate junior. Next: why do amerikans complain about pain more than any people? Why do you constantly complain about what you've read about Americans? Have you ever even fucking been here? I've personally known well over ten thousand (easily) Americans. Have you? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? Have you ever even fucking done ANY of them? I have. What do you wish to know? Until then, please spare me your uninformed words which I guess are your 'opinions', for whatever that's worth, which ain't much. RC
Rowan Cocoan · 2021-06-21T08:28:44Z
smertzasmert I said, 'Now you're merely dealing with semantics . and you reply with now you are insane... Well, from around here, we'd say something like: You need to work on your art of debate junior. Next: why do amerikans complain about pain more than any people? Why do you constantly complain about what you've read about Americans? Have you ever even fucking been here? I've personally known well over ten thousand (easily) Americans. Have you? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? Have you ever even fucking done ANY of them? I have. What do you wish to know? Until then, please spare me your uninformed words which I guess are your 'opinions', for whatever that's worth, which ain't much. RC
why do amerikans complain about pain more than any people?
why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples?
parzival · Rowan Cocoan · 2021-06-21T08:40:42Z
Rowan Cocoan · 2021-06-21T08:51:33Z smertzasmert Oh, why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? 1) WTF is a 'legal' psychotropic? Where and when? I'd guess that SSRI's (which you are apparently referencing) have not been approved in many countries. Here's some proven physically addictive psychotropic drugs: caffeine. (You stop using it, you get a headache.) Alcohol. (Drink too much and you can get the DT's.) Here's one of countless that is more psychologically addictive: BAKING SODA! (People who love burping have gotten so addicted to water mixed with NaHCO3 that if they went cold turkey* they would have died.) why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? 2) Maybe you've never heard of our 'blues' music? Created by our poor oppressed brethren? Argh! RC *It's an Americanism. Look it up. Ps, Kid, I don't want to, but I'm about to give up on you. rc
Rowan Cocoan · 2021-06-21T08:51:33Z
smertzasmert Oh, why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? 1) WTF is a 'legal' psychotropic? Where and when? I'd guess that SSRI's (which you are apparently referencing) have not been approved in many countries. Here's some proven physically addictive psychotropic drugs: caffeine. (You stop using it, you get a headache.) Alcohol. (Drink too much and you can get the DT's.) Here's one of countless that is more psychologically addictive: BAKING SODA! (People who love burping have gotten so addicted to water mixed with NaHCO3 that if they went cold turkey* they would have died.) why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? 2) Maybe you've never heard of our 'blues' music? Created by our poor oppressed brethren? Argh! RC *It's an Americanism. Look it up. Ps, Kid, I don't want to, but I'm about to give up on you. rc
why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade?
why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow?
parzival · Rowan Cocoan · 2021-06-21T08:56:18Z
Rowan Cocoan · 2021-06-21T08:58:28Z parzival I detect (or recall) that you gave up on Smertz a long long time ago... [ Link ] Bad news on the doorstep. . . the day that . . . I came across another smertzitism . . RC
Rowan Cocoan · 2021-06-21T08:58:28Z
parzival I detect (or recall) that you gave up on Smertz a long long time ago... [ Link ] Bad news on the doorstep. . . the day that . . . I came across another smertzitism . . RC
parzival · 2021-06-21T09:06:00Z Rowan Cocoan Yeah! American Pie... I was nearly one years old when Buddy Holly met his end...
parzival · 2021-06-21T09:06:00Z
Rowan Cocoan Yeah! American Pie... I was nearly one years old when Buddy Holly met his end...
Nada · 2021-06-21T21:58:01Z smertzasmert What’s with the “K” in American?
smertzasmert What’s with the “K” in American?
Rowan Cocoan · 2021-06-21T23:48:53Z Nada He initly wld typ stuff like this, like some early (at least as I understood it here) form of texting. He's almost never used capitals and ALWAYS (as far as I'm aware) described us all as 'amerikans'. He further lumps us all together, all the time, 24/7/366. He routinely would initially post series of quotes from people who I would consider 'modern Marxists'. One IRRITATING thing that he's routinely done is posting such quotes which the originals of say America or American etc. WITH the C changed to a K. I had some hopes for the kid but . . . I would LOVE to read a reply from him that addresses what I pointed out in my above reply to him, but I don't expect it. Oh well. RC
Rowan Cocoan · 2021-06-21T23:48:53Z
Nada He initly wld typ stuff like this, like some early (at least as I understood it here) form of texting. He's almost never used capitals and ALWAYS (as far as I'm aware) described us all as 'amerikans'. He further lumps us all together, all the time, 24/7/366. He routinely would initially post series of quotes from people who I would consider 'modern Marxists'. One IRRITATING thing that he's routinely done is posting such quotes which the originals of say America or American etc. WITH the C changed to a K. I had some hopes for the kid but . . . I would LOVE to read a reply from him that addresses what I pointed out in my above reply to him, but I don't expect it. Oh well. RC
smertzasmert · 2021-06-21T08:13:31Z Rowan Cocoan now you are insane...why are north amerikan definitions of diseases not employed in Europe? why has the DSM been changed 8 times and is not employed in civilized nations? why have definitions of gender dysphoria changed? sociopathy? anti-social personality dx? psychopathy changed in the DSM numerous times? why do amerikans complain about pain more than any people? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? why do only north amerikans have this as Geoffrey Gorer wrote "a patterned courtship ritual described as dating where they are required to meet in public spaces several occasions before they copulate"?
smertzasmert · 2021-06-21T08:13:31Z
Rowan Cocoan now you are insane...why are north amerikan definitions of diseases not employed in Europe? why has the DSM been changed 8 times and is not employed in civilized nations? why have definitions of gender dysphoria changed? sociopathy? anti-social personality dx? psychopathy changed in the DSM numerous times? why do amerikans complain about pain more than any people? why do north amerikans consume more coke, heroin marijuana, meth, hallucinogens per capita than all peoples? why have amerikans consumed 80+% of the legal psychotropics and 66% of the anti-depressants on earth in the past decade? why do only north amerikans consider a color to be depression---blue? or another color to be cowardice, yellow? why do only north amerikans have this as Geoffrey Gorer wrote "a patterned courtship ritual described as dating where they are required to meet in public spaces several occasions before they copulate"?
LindaMay · 2021-06-21T13:55:27Z smertzasmert you forgot, bodyshapes.
LindaMay · 2021-06-21T13:55:27Z
smertzasmert you forgot, bodyshapes.
BlackCartouche · 2021-06-21T18:45:13Z Don't know if this has already been linked elsewhere on SOTT, but Science is now saying human cells can write RNA sequences into DNA... [ Link ] Source: Thomas Jefferson University Summary: In a discovery that challenges long-held dogma in biology, researchers show that mammalian cells can convert RNA sequences back into DNA , a feat more common in viruses than eukaryotic cells. It would strongly suggest the mRNA jab can alter our DNA after all - just like them 'spiracy theory-ists sed all along! Thought it worth copy/pasting the whole article here... Cells contain machinery that duplicates DNA into a new set that goes into a newly formed cell. That same class of machines, called polymerases, also build RNA messages, which are like notes copied from the central DNA repository of recipes, so they can be read more efficiently into proteins. But polymerases were thought to only work in one direction DNA into DNA or RNA. This prevents RNA messages from being rewritten back into the master recipe book of genomic DNA. Now, Thomas Jefferson University researchers provide the first evidence that RNA segments can be written back into DNA, which potentially challenges the central dogma in biology and could have wide implications affecting many fields of biology. "This work opens the door to many other studies that will help us understand the significance of having a mechanism for converting RNA messages into DNA in our own cells," says Richard Pomerantz, PhD, associate professor of biochemistry and molecular biology at Thomas Jefferson University. "The reality that a human polymerase can do this with high efficiency, raises many questions." For example, this finding suggests that RNA messages can be used as templates for repairing or re-writing genomic DNA. The work was published June 11th in the journal Science Advances. Together with first author Gurushankar Chandramouly and other collaborators, Dr. Pomerantz's team started by investigating one very unusual polymerase, called polymerase theta. Of the 14 DNA polymerases in mammalian cells, only three do the bulk of the work of duplicating the entire genome to prepare for cell division. The remaining 11 are mostly involved in detecting and making repairs when there's a break or error in the DNA strands. Polymerase theta repairs DNA, but is very error-prone and makes many errors or mutations. The researchers therefore noticed that some of polymerase theta's "bad" qualities were ones it shared with another cellular machine, albeit one more common in viruses -- the reverse transcriptase. Like Pol theta, HIV reverse transcriptase acts as a DNA polymerase, but can also bind RNA and read RNA back into a DNA strand. In a series of elegant experiments, the researchers tested polymerase theta against the reverse transcriptase from HIV, which is one of the best studied of its kind. They showed that polymerase theta was capable of converting RNA messages into DNA, which it did as well as HIV reverse transcriptase, and that it actually did a better job than when duplicating DNA to DNA. Polymerase theta was more efficient and introduced fewer errors when using an RNA template to write new DNA messages, than when duplicating DNA into DNA, suggesting that this function could be its primary purpose in the cell. The group collaborated with Dr. Xiaojiang S. Chen's lab at USC and used x-ray crystallography to define the structure and found that this molecule was able to change shape in order to accommodate the more bulky RNA molecule -- a feat unique among polymerases. "Our research suggests that polymerase theta's main function is to act as a reverse transcriptase," says Dr. Pomerantz. "In healthy cells, the purpose of this molecule may be toward RNA-mediated DNA repair. In unhealthy cells, such as cancer cells, polymerase theta is highly expressed and promotes cancer cell growth and drug resistance. It will be exciting to further understand how polymerase theta's activity on RNA contributes to DNA repair and cancer-cell proliferation."
BlackCartouche · 2021-06-21T18:45:13Z
Don't know if this has already been linked elsewhere on SOTT, but Science is now saying human cells can write RNA sequences into DNA... [ Link ] Source: Thomas Jefferson University Summary: In a discovery that challenges long-held dogma in biology, researchers show that mammalian cells can convert RNA sequences back into DNA , a feat more common in viruses than eukaryotic cells. It would strongly suggest the mRNA jab can alter our DNA after all - just like them 'spiracy theory-ists sed all along! Thought it worth copy/pasting the whole article here... Cells contain machinery that duplicates DNA into a new set that goes into a newly formed cell. That same class of machines, called polymerases, also build RNA messages, which are like notes copied from the central DNA repository of recipes, so they can be read more efficiently into proteins. But polymerases were thought to only work in one direction DNA into DNA or RNA. This prevents RNA messages from being rewritten back into the master recipe book of genomic DNA. Now, Thomas Jefferson University researchers provide the first evidence that RNA segments can be written back into DNA, which potentially challenges the central dogma in biology and could have wide implications affecting many fields of biology. "This work opens the door to many other studies that will help us understand the significance of having a mechanism for converting RNA messages into DNA in our own cells," says Richard Pomerantz, PhD, associate professor of biochemistry and molecular biology at Thomas Jefferson University. "The reality that a human polymerase can do this with high efficiency, raises many questions." For example, this finding suggests that RNA messages can be used as templates for repairing or re-writing genomic DNA. The work was published June 11th in the journal Science Advances. Together with first author Gurushankar Chandramouly and other collaborators, Dr. Pomerantz's team started by investigating one very unusual polymerase, called polymerase theta. Of the 14 DNA polymerases in mammalian cells, only three do the bulk of the work of duplicating the entire genome to prepare for cell division. The remaining 11 are mostly involved in detecting and making repairs when there's a break or error in the DNA strands. Polymerase theta repairs DNA, but is very error-prone and makes many errors or mutations. The researchers therefore noticed that some of polymerase theta's "bad" qualities were ones it shared with another cellular machine, albeit one more common in viruses -- the reverse transcriptase. Like Pol theta, HIV reverse transcriptase acts as a DNA polymerase, but can also bind RNA and read RNA back into a DNA strand. In a series of elegant experiments, the researchers tested polymerase theta against the reverse transcriptase from HIV, which is one of the best studied of its kind. They showed that polymerase theta was capable of converting RNA messages into DNA, which it did as well as HIV reverse transcriptase, and that it actually did a better job than when duplicating DNA to DNA. Polymerase theta was more efficient and introduced fewer errors when using an RNA template to write new DNA messages, than when duplicating DNA into DNA, suggesting that this function could be its primary purpose in the cell. The group collaborated with Dr. Xiaojiang S. Chen's lab at USC and used x-ray crystallography to define the structure and found that this molecule was able to change shape in order to accommodate the more bulky RNA molecule -- a feat unique among polymerases. "Our research suggests that polymerase theta's main function is to act as a reverse transcriptase," says Dr. Pomerantz. "In healthy cells, the purpose of this molecule may be toward RNA-mediated DNA repair. In unhealthy cells, such as cancer cells, polymerase theta is highly expressed and promotes cancer cell growth and drug resistance. It will be exciting to further understand how polymerase theta's activity on RNA contributes to DNA repair and cancer-cell proliferation."
Source: Thomas Jefferson University Summary: In a discovery that challenges long-held dogma in biology, researchers show that mammalian cells can convert RNA sequences back into DNA , a feat more common in viruses than eukaryotic cells.
Cells contain machinery that duplicates DNA into a new set that goes into a newly formed cell. That same class of machines, called polymerases, also build RNA messages, which are like notes copied from the central DNA repository of recipes, so they can be read more efficiently into proteins. But polymerases were thought to only work in one direction DNA into DNA or RNA. This prevents RNA messages from being rewritten back into the master recipe book of genomic DNA. Now, Thomas Jefferson University researchers provide the first evidence that RNA segments can be written back into DNA, which potentially challenges the central dogma in biology and could have wide implications affecting many fields of biology. "This work opens the door to many other studies that will help us understand the significance of having a mechanism for converting RNA messages into DNA in our own cells," says Richard Pomerantz, PhD, associate professor of biochemistry and molecular biology at Thomas Jefferson University. "The reality that a human polymerase can do this with high efficiency, raises many questions." For example, this finding suggests that RNA messages can be used as templates for repairing or re-writing genomic DNA. The work was published June 11th in the journal Science Advances. Together with first author Gurushankar Chandramouly and other collaborators, Dr. Pomerantz's team started by investigating one very unusual polymerase, called polymerase theta. Of the 14 DNA polymerases in mammalian cells, only three do the bulk of the work of duplicating the entire genome to prepare for cell division. The remaining 11 are mostly involved in detecting and making repairs when there's a break or error in the DNA strands. Polymerase theta repairs DNA, but is very error-prone and makes many errors or mutations. The researchers therefore noticed that some of polymerase theta's "bad" qualities were ones it shared with another cellular machine, albeit one more common in viruses -- the reverse transcriptase. Like Pol theta, HIV reverse transcriptase acts as a DNA polymerase, but can also bind RNA and read RNA back into a DNA strand. In a series of elegant experiments, the researchers tested polymerase theta against the reverse transcriptase from HIV, which is one of the best studied of its kind. They showed that polymerase theta was capable of converting RNA messages into DNA, which it did as well as HIV reverse transcriptase, and that it actually did a better job than when duplicating DNA to DNA. Polymerase theta was more efficient and introduced fewer errors when using an RNA template to write new DNA messages, than when duplicating DNA into DNA, suggesting that this function could be its primary purpose in the cell. The group collaborated with Dr. Xiaojiang S. Chen's lab at USC and used x-ray crystallography to define the structure and found that this molecule was able to change shape in order to accommodate the more bulky RNA molecule -- a feat unique among polymerases. "Our research suggests that polymerase theta's main function is to act as a reverse transcriptase," says Dr. Pomerantz. "In healthy cells, the purpose of this molecule may be toward RNA-mediated DNA repair. In unhealthy cells, such as cancer cells, polymerase theta is highly expressed and promotes cancer cell growth and drug resistance. It will be exciting to further understand how polymerase theta's activity on RNA contributes to DNA repair and cancer-cell proliferation."
Rowan Cocoan · 2021-06-21T23:56:00Z BlackCartouche Oh, you must be one of them thar 'spiracy theory-ists! (Love those extra syllables: 'the or ree ists' ! ) RC
Rowan Cocoan · 2021-06-21T23:56:00Z
BlackCartouche Oh, you must be one of them thar 'spiracy theory-ists! (Love those extra syllables: 'the or ree ists' ! ) RC
parzival · 2021-06-22T01:16:25Z BlackCartouche Thanks for your post, summation, and link. The upside is the cure for out of control cell proliferation. The downside is weaponising polymerase theta...
parzival · 2021-06-22T01:16:25Z
BlackCartouche Thanks for your post, summation, and link. The upside is the cure for out of control cell proliferation. The downside is weaponising polymerase theta...